The following list of resources includes a selection of automated tools which can be used for accessibility checks. These tools are extremely valuable and useful, but can give false positives and negatives so please do not solely rely on them.
Easy instructions
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W3C Web Accessibility Initiative: strategies, guidelines and resources for web accessibility
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W3C Web Accessibility Perspectives (Videos): brief explanations of accessibility features and their benefits for everyone
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W3C Tips for Getting Started with Web Accessibility: easy to do things for different types of audiences
Advanced instructions
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WebAIM WCAG 2.0 Checklist: checklist presenting recommendations for implementing HTML-related principles and techniques for those seeking WCAG 2.0 conformance
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W3C Web Accessibility Tutorials: more in-depth (but practical) tutorials on how to implement specific accessibility features and concepts
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W3C markup validation service: checks the markup validity of Web documents in HTML, XHTML, SMIL, MathML, etc.
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WAVE: web accessibility evaluation tool
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Functional Accessibility Evaluator (FAE): website accessibility evaluation tool
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The Paciello Group Colour Contrast Analyser: provides a pass/fail assessment against WCAG 2.0 colour contrast success criteria and simulates certain visual conditions to demonstrate how web content appears to people with less than 20/20 vision.
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TAW: WCAG 1.0 and 2.0 and mobileOK web checker (in English Spanish, Catalan and Galician)
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ACHECKER: web accessibility checker
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Total Validator: all-in-one (X)HTML validator, accessibility validator, spell checker and broken links checker
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AccessMonitor: web accessibility checker (in Portuguese)
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Examinator: web accessibility checker (in Spanish)
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MSF&W colour contrast ratio calculator: to check the contrast of colours on web pages
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Pause, Stop, Hide: guidance on mechanism for users to pause, stop or hide animated content
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TRACE Photosensitive Epilepsy Analysis Tool (PEAT): free, downloadable resource to identify seizure risks in web content and software